Paper chromatography is a modern method used separate mixtures. Paper chromatography uses paper as the stationary phase and a liquid solvent as the mobile phase.
The purposes of this lab were to separate mixture of food colorings into their components and to identify the components of mixtures by means of their Rf values.
Materials we need: 3 large testtubes, 3 Erlenmyer flasks, 3 strips of 22 chromatography papers, food colourings, a pencil, a ruler and a calculator
The procedures of this lab would be:
Part I
- draw a line across each strips of chromatography paper 4.0 cm from one end, then trim the end of the strip
- place 2.0 cm deep water in each testtube
- spot one strip with the color, label, and insert the strip in test tube A
- observe what happens
- identify two front on the paper after the next 10 min
- start part II as the movement slow down
- after 20 min, remove the strip and immediately draw a pencil line across the top edge
- measure d2 and d1 and record in Table 1 and 2
- clean up
- spot the second strip of CP with a sample of green food coloring, and spot the third CP with an unknown mixture
- insert the strips and follow the same procedures in part 1
- record data
So, Here's a great video about color chromatography. Watch it and you would know more about this lab!!
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